Blue light receptor
| part
| plasmid
| description
| status
| μl left
| concentration
| date
|
| MC4100 | / | strain of E.coli | | | | july 24
|
| 2171 | / | forward primer for | | 90µl | 20μM | july 24
|
| 2172 | / | reverse primer for | | 90µl | 20μM | july 24
|
| 2171 | / | forward primer for | | | 100μM | july 24
|
| 2172 | / | reverse primer for | | | 100μM | july 24
|
| 2260 | / | forward primer for | | 45µl | 20μM | aug 10
|
| 2261 | / | reverse primer for | | 45µl | 20μM | aug 10
|
| 2260 | / | forward primer for | | | 100μM | aug 10
|
| 2261 | / | reverse primer for | | | 100μM | aug 10
|
| | pSB1A2 | GFP + rbs + terminator | purified | | | july 29
|
| | pSB1A2 | GFP + rbs + terminator | restricted with EcoRI and XbaI | | | july 29
|
| | / | BLR promoter region | restricted with EcoRI | | | july 29
|
| | / | BLR promoter region | 4 microcentrifuge tubes | | | july 30
|
| | / | BLR promoter region | 1 microcentrifuge tube | 28μl conc 102,6 ng/μl | aug 10
|
Key/antikey/lock
| part
| plasmid
| description
| status
| μl left
| concentration
| date
|
| / | GFP with LVA tag | purified | | | july 28
|
| / | tetR promoter | purified | | | july 28
|
vanillin synthesis
| part
| plasmid
| description
| status
| μl left
| concentration
| date
|
| pSB1A2 | ech + rbs | purified plasmid | | 108,8 ng/µl | August 4
|
| pSB1A2 | fcs + rbs | purified plasmid | | 113,9 ng/µl | August 4
|
| pSB1A2 | Sam5 + rbs | received from Edinburgh | | | August 3
|
| pSB1A2 | Sam5 + rbs, PstI site removed | purified plasmid | | 101,3 ng/µl | August 5
|
| pSB1A2 | Sam8 + rbs | purified plasmid | | 95,7 ng/µl | August 5
|
| pSB1A2 | Sam8 | purified plasmid | | 81,5 ng/µl | August 5
|
| pSB1A2 | COMT | purified plasmid | | 122,9 ng/µl | August 5
|
| pSB1A2 | COMT + rbs | plasmid from registry | | | August 6
|
| pSB1A2 | ech + rbs | purified digest | | 16,9 ng/µl | August 6
|
| | fcs + rbs | purified digest | | 16,6 ng/µl | August 6
|
| pSB1A2 | sam5 + rbs | purified digest | | 3,1 ng/µl | August 7
|
| | sam8 + rbs | purified digest | | 3,4 ng/µl | August 7
|
vanillin receptor
| part
| plasmid
| description
| status
| μl left
| concentration
| date
|
|-
|||pSB1A2||ech + rbs||purified plasmid||||108,8 ng/µl||August 4
|-
|||pSB1A2||fcs + rbs||purified plasmid||||113,9 ng/µl||August 4
|-
|||pSB1A2||Sam5 + rbs||received from Edinburgh||||||August 3
|-
|||pSB1A2||Sam5 + rbs, PstI site removed||purified plasmid||||101,3 ng/µl||August 5
miscellaneous
| part
| plasmid
| description
| status
| μl left
| concentration
| date
|
| dNTP mix | | ATP, GTP, TTP, CTP | | 94 | 10μM | aug 10
|
| pSB1A2 | medium constitutieve promoter | | | | july 23
|
| ampicilline | | | | | |
|
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