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Modelling
From 2009.igem.org
To find
PDB files of LOV domain, TrpR repressor => http://www.rcsb.org/pdb/home/home.do
To do
- Set up day of starting modeling, do a planning for modeling => see with Matteo Dal Peraro ----> will be setted up as soon as the exams are over
- Model allosteric interactions between LOVTAP & TrpR:
What will be done:
- -> model of LOVTAP in dark phase
- -> model of LOVTAP in light phase
- -> characterize how the J-alpha helix changes
- Model the fusion of LOV domain with other transcription factors
- Do a mutational assay to change specificity of LOVTAP:
- Direct mutational assay: Insert mutation in specific sites thanks to the known structure of LOVTAP (already modeled on computer)
- Indirect mutational assay: Random mutations, then selection of the "right" protein according to a set of selected conditions
To envisage
- Molecular mutationnal assay
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