Virginia Commonwealth/4 August 2009

Revision as of 18:27, 13 August 2009 by Trentay (Talk | contribs)

Tuesday 4 August 2009

Maria and Afton

Overnight culture of I1352 plated on AMP:
- Sample taken from plate after PSB4C5 ligation attempt successfully grew expressing RFP. The color of the sample was pink
- Sample taken from plate after ligation with itself did not grow at all, and expressed no RFP
There is a possibility the pSB4C5 backbone may be a bad part
- previous years it has not functioned properly
- Gel electrophoresis indicates DNA Gel Purification may be necessary
Possible inefficiency of BioBrick enzymes may have come from repeated freezing and thawing
- They will be aliquoted into smaller amounts
Gel Electrophoresis Results

From Left to Right: 100bp Ladder, Bioline Hyperladder, I1352(Digest), I1352(Miniprep), pSB4C5(Digest), pSB4C5(Miniprep)

Trentay 22:25, 4 August 2009 (UTC)

Maria and Afton

Trentay 22:11, 4 August 2009 (UTC)

New BioBrick Assembly Kit came in and the following were aliquoted into their respective amounts


Name	Amount/Tube	# Tubes	Extra in Stock
XbaI	10 µL	15	No
SpeI	5 µL	10	No
PstI	10 µL	25	Yes
EcoRI-HF	10 µL	25	Yes
BSA	5 µL	8	Yes
T4 Ligase Buffer	10 µL	25	Yes
T4 Ligase	10 µL	8	No

To show how many times a tube has been thawed and refrozen, a hash mark will be placed on the side of the tube, each time it is thawed as a reference for others to see

Trentay 15:24, 5 August 2009 (UTC)

Maria and Afton

Gel Electrophoresis was run
- pSB4C5 and I1352 digested and undigested (right after Miniprep) were run on a gel and results showed excessive bands in the undigested DNA. DNA Gel Purification will be done on future Miniprepped DNA
pSB1C3 w/ P1010 was removed from Well 5E on Plate 1 of the Distribution Kit and resuspended in 10µL of TE Buffer.
Transformation was done using DB 3.1

Trentay 22:33, 4 August 2009 (UTC)