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Modelling
From 2009.igem.org
To find
PDB files of LOV domain, TrpR repressor => [http://www.rcsb.org/pdb/home/home.do PDB database]
To do
OK - Set up day of starting modeling, do a planning for modeling => see with Matteo Dal Peraro ----> will be setted up as soon as the exams are over
Ask Matteo to send all the required starting papers, files, and links to tutorials BEFORE the end of the exams (around midjune)
- Model allosteric interactions between LOVTAP & TrpR:
- What will be done:
- -> model of LOVTAP in dark phase
- -> model of LOVTAP in light phase
- -> characterize how the J-alpha helix changes
We will follow the following article protocol:
Freddolino, P.L., Dittrich M., Schulten K., Dynamic Switching Mechanisms in LOV1 and LOV2 Domains of Plant Phototropins. Biophysical Journal, 91, 3630-3639, 2006 ([http://www.ncbi.nlm.nih.gov/sites/entrez?db=pmc&cmd=search&term=PMC1630464 Pubmed])
- Model the fusion of LOV domain with other transcription factors </b>
To envisage
- Molecular mutationnal assay
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