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CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
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CHINMOYEE
Three Gene Repressilator Presentation
Presented Ander's Modeling examples of the Three Gene Repressilator .
Looked at the Optimization Toolbox:
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EMILY
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
Team meeting
No lab experiments were performed
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MANDY
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PATRICK
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PRIMA
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STEFAN
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VICKI
Purification of gradient PCR product
Purpose: to purify the LuxOD47A BBk that was produced yesterday in the gradient PCR
Protocol: Please refer to the protocol page. Concentrations were measured after the product was purified.
Restriction digest of LuxOD47A BBk (insert) and psB1AC3 (vector)
Purpose: The LuxOD47A BBk DNA from yesterday’s gradient PCR is in linear form. This will prepare the ends so that it can be inserted into a BioBrick vector.
Protocol:
This is the restriction digest component of the construction protocol, which we describe on our protocol page. We attempted the construction with 2 different sets of enzymes: 6 tubes with cuts at XbaI and PstI , and 6 tubes with cuts at EcoRI and PstI. Both were prepared in REact 2 buffer. Once prepared, the tubes were left in the water bath at 37 degrees C for 6.5 hours; heatshocked at 65 degrees C on a heating block to deactivate the restriction enzymes in the tube; and placed in the -20 degrees C freezer.
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