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CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
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CHINMOYEE
CLASS
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EMILY
Verification Colony PCR of BBK LuxOD47E in psBA1AC3 Vector
- Performed colony PCR again with the proper PCR Buffer this time. PCR products were visualized on a 1% agarose gel run at 120 V with Generuler 1.0 KB+ DNA Ladder. Lanes 1-7 are LuxOD47E in psB1AC3 cut with XbaI / PstI. Lane 8 is a negative control with only Mastermix + ddH2O.
- See gel photo below.
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
Descriptive Title of What You're Doing
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KEVIN
1. Glycerol Stock
Glycerol stocks of R0040, B0015 J13002, and Pqrr4 were made in order to preserve them long term. The addition of glycerl is important because otherwise the cellular membrane would shear.
2. Plasmid Isolation
To obtain more isolated R0040, B0015, J1302, and Pqrr4, miniprep (plasmid isolation) was done on them.
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MANDY
Descriptive Title of What You're Doing
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PATRICK
Descriptive Title of What You're Doing
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PRIMA
Marketing & Sponsorship
After constantly following up with companies over the past few weeks, I successfully recruited Alberta Research Council to sponsor the University of Calgary's iGEM team. They have generously donated %250 dollars this year.
We also edited the June newsletter and discussed it with the rest of the marketing team prior to sending it to all the companies we had contacted (including those who have declined our proposal).
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STEFAN
Descriptive Title of What You're Doing
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VICKI
Sequencing of what I hope will be LuxOD47A BBK
I prepared a sample of the recently-formed-and-colony-PCR'd-and-NotI-restriction-digested LuxOD47A BBk for sequencing. This was done with BBk sequencing primers, in accordance with the sequencing protocol.
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