Template:Team:KULeuven/26 August 2009/VanillinProduction

From 2009.igem.org

Revision as of 09:13, 26 August 2009 by K3n (Talk | contribs)
  • Miniprep of fluid cultures

Nanodrop results:

Part concentration (ng/μl) 260/280 λ
SamS I 53,9 1,87
SamS II 39,4 2,16
SamS III 57,1 2,07
EF I 76,8 1,94
EF II 83,4 2,04
EF III 61,5 2,01
  • Restriction:

-We performed 2 simultaneous digests: one to verify the length of the product (Restriction2Test) using SAMS I and EF III and another to prepare for ligation (Restriction4Real) with promotor and terminator using SAMS III and EF II

-We cut with the 2 enzymes separately, allowing a one hour incubation between

  • Terminator was also digested
Retrieved from "http://2009.igem.org/Template:Team:KULeuven/26_August_2009/VanillinProduction"