Template:Team:KULeuven/1 September 2009/VanillinProduction

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Revision as of 06:35, 3 September 2009 by K3n (Talk | contribs)
  • SAMS I B RD is used for ligation with TER II RD
    • Since we want to avoid gel purification, we use the direct sample from the restriction digest
    • The restriction enzymes are denatured by keeping the sample at 85°C for 20 minutes
vector insert
Terminator SAMS
2000 bp 3070 bp
50 ng 280 ng
10 μl 16 μl
  • The genes fcs and ech are individually cut with the four restriction enzymes
EcoR1 fcs RD-E
Spe1 fcs RD-S
Pst1 fcs RD-P
Xba1 fcs RD-X
EcoR1 Ech RD-E
Spe1 Ech RD-S
Pst1 Ech RD-P
Xba1 Ech RD-X
  • Results on gel show that all the genes have the same length and display 1 line.
  • SAMS I A RD is put on gel, to be used for gel extraction and a part of SAMS I B RD is put on gel to check the length.
    • No signal... grrr...
  • Replated colonies from the original SAMS plate from 24/8
    • Took 4 individual colonies and plated them on 4 corners of a petri dish
  1. the 4 genes(sam5, sam8, ech, fcs) where taken from the original DNA that was sent, electroporated and plated. This in order to make a glycerol stock.