Template:Team:KULeuven/3 September 2009/BlueLightReceptor

From 2009.igem.org

Revision as of 15:09, 3 September 2009 by AnneV (Talk | contribs)
  1. a restriction digest on with EcoRI and SpeI was performed. this will cut the promotor out of its vector () which has a RFP reporter gene. we will put (cut with EcoRI and SpeI) in this vector. This way we will get a construct where RFP will be produced dependend on our blue light promotor. we can then compare this with the intensity of RFP produced by .
  2. glycerol stock was made from LigA and . they are stored in the -80°C in S&P F8 67-68 and 69-70.
  3. Ecoli strain DB3.1 cells were made competent for electroporesis.
  4. gel electorforesis and extraction on
Retrieved from "http://2009.igem.org/Template:Team:KULeuven/3_September_2009/BlueLightReceptor"