Team:UNICAMP-Brazil/Notebooks/August 15
From 2009.igem.org
YeastGuard: RBS and CFP plasmid digestion
- Today we started the construction of our first biobrick. After quantificate the mini-prep's products we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter.
- After the digestion we isolated the target bands from the agarose gel, according to the protocol X.
- The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today.
Raíssa