"
Uppsala-Sweden/6 September 2009
From 2009.igem.org
Contents |
Moving ON to fride
ON cultures for the lux system were moved to the iGEM fridge for MiniPrep tomorrow
Checking Plates
All plates show colonoies. After picking colonies -> fridge. --Karl.brune 23:08, 5 September 2009 (UTC)
Picking Colonies
I picked colonies from plates into 10µl selective media. --Karl.brune 23:08, 5 September 2009 (UTC) 5 Colonies of pirA (AC3) -> pirA-X 5 Colonies of b0034-pirA (AC3) -> RA-X 5 Colonies of B0034-Yadh2-B0015 (AC3) -> RYB-X 5 Colonies of B0015 (AC3) - T-X 5 Colonies of R0011-B0034 (AK3) -> RR-X
3 Colonies of E1A1-B0015 (AK3) -> E1A1X 3 Colonies of E1A2-B0015 (AK3) -> E1A2X 3 Colonies of E1A3-B0015 (AK3) -> E1A3X 3 Colonies of E1A4-B0015 (AK3) -> E1A4X
3 Colonies of E1B1-B0015 (AK3) -> E1B1X 3 Colonies of E1B2-B0015 (AK3) -> E1B2X 3 Colonies of E1B3-B0015 (AK3) -> E1B3X 3 Colonies of E1B4-B0015 (AK3) -> E1B4X
3 Colonies of E21-B0015 (AK3) -> E21X 3 Colonies of E22-B0015 (AK3) -> E22X 3 Colonies of E23-B0015 (AK3) -> E23X 3 Colonies of E24-B0015 (AK3) -> E24X
X= # of colony
--Karl.brune 23:08, 5 September 2009 (UTC)
Colony PCR
TAQ colony PCR was performed.
10µl reaction, standart mix with verfication primer VF2 and VR.
Initial Denat: 1min 30sec @94°C
40Cylces
Denaturation: 45 sec @94°C
Annealing: 45 sec @50°C
Elongation: 1min 45sec @ 72°C
Return to Denat.
Final Elongation: 10min @72°C
Infinite storage at 4°C
Colony tubes were moved to fridge after taking template for the PCR
--Karl.brune 23:08, 5 September 2009 (UTC)
