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File:Chiba Labwork 09Sep09.jpg
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0800 main culture
divided injection 12.5ml of the culture to 10 50ml centrifuging tubes
WASH 3 times
Add 12.5ml new culture
Centrifuge the 1st tube 3000rpm 5min 20℃ and pick the upernatant
Do same operation every 15min to 2nd~10th tubes
after all, mix the supernatant and solid media at 1:1
make 10 concentration varieties of solid media
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 05:03, 10 September 2009 | | 1,600×2,329 (292 KB) | Yoshimi (Talk | contribs) |
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