Template:Team:KULeuven/11 August 2009/VanillinReceptor

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  • We also received another strain of Agrobacterium. We brought this new strain in culture.
  • To make things clear the genes from the first Agrobacterium will from now on be called A,B,G and R for respectively virA, promotor region virB , virG and rpoA. For the second Agrobacterium we will use W,X,Y and Z to indicate the same genes.
  • We achieved good results for genes B,G and R. There were clear white colonies after plating the competent cells with the TOPO vector on X-gal agar plates with ampicillin. We isolated the good colonies again.
  • There was no result for A. This was probably due to a fault with the competent cells. So we started TOPO transformation by heat shock all over for A.
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