Team:Chiba/Notebook/Calendar/17 September 2009

From 2009.igem.org

Revision as of 05:24, 23 September 2009 by Makoto (Talk | contribs)

>Go to the Notebook page

(16_September_2009 <|>18_September_2009)

Mini Prep

10:30~

  1. I731012(1930bp) pSB1A2(2079bp)
  2. K091134(2048bp) pSB1AK3(3189bp)
  3. S03956(1041bp) pSB1A2(2079bp)
  4. K091118(1985bp) pSB1A2(2079bp)

Digestion Test

14:30~

Double Digestion

volume (uL)
DNA 3 (300ng)
EcoRI 0.20 (4U)
PstI 0.20 (4U)
Buffer(10x) 1
BSA(10x) 1
dW 4.6
total 10



Single Digestion

volume (uL)
DNA 3 (300ng)
EcoRI 0.20 (4U)
Buffer(10x) 1
dW 5.8
total 10


Gel

agar(low electro-osmosis)---0.7%(W)---0.42 g

TAE---60 ml


Energization

135V 27min


Result

from left to right

marker

Lab Notebook

Chiba-Labwork-14Sep09.jpg Chiba-Labwork-17Sep09.jpg Chiba-Labwork-17Sep09-2.jpg Chiba-Labwork-17Sep09-3.jpg

Chiba-Labwork-17Sep09-4.jpg Chiba-Labwork-17Sep09-5.jpg Chiba-Labwork-17Sep09-6.jpg

Retrieved from "http://2009.igem.org/Team:Chiba/Notebook/Calendar/17_September_2009"