Team:Chiba/Notebook/Calendar/24 September 2009

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Examine limit of AHL generation

Yesterday's operation is here.


  • Today's operation

22:00

We measured OD.

OD600=1.98


Then, we spun down this culture solution and add 12.5 mL of flesh LB-Amp.


22:10

We add 12.5 μL of IPTG to the culture solution and started culture at 37 degrees Celsius.

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