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Imperial College London/Notebook/11 September 2009
From 2009.igem.org
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Contents |
Testing
Diauxic growth 11th Sep
Protocol considerations
Using spectrophotometer at 600nm Blanks are all the relevant carbon sources, but subsequently realised that all blanks were roughly the same (within 0.03) Therefore, subsequent readings= blank+ 6 sample
Overnight culture had OD of 1.64. 800ul was put into each sample of 40ml~ 0.033 OD ie same initial OD Grown in 6 separate 50ml falcon tubes and shaking in incubator at 28 degrees Celsius 1ml taken out at 1 hr intervals from the falcon tube and placed into spectrophotometer
Results
Conclusions
Suggestions/Improvements
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