From 2009.igem.org
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March 2009
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April 2009
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May 2009
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November 2009
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Week from June 15th, to June 21st, 2009
June, 15th
June, 16th
June, 17th
Trasformation of Ligations
A5: BBa_Q0040 with BBa_I732017 (RBS-TETR-PTET-RBS-LACZ-TT)
A6: BBa_J23100 with BBa_Q0040 (PCON-RBS-TETR-TT-PTET )
- 1 ul A5 in Top10 Competent
- 1 ul A6 in Top10 Competent
Mix 80 ul of SOC with 20 ul X-GAL (40 mg/ml) and infect LB+Amp Agar plates.
- Add 250 ul SOC in 50 ul A5+ Top10
- Add 250 ul SOC in 50 ul A6+ Top10
Incubated 1 hr 37°C (falcons with shaking 220 rpm)
Infect:
- X-GAL plate with 200 ul A5
- X-GAL plate with 100 ul A5
- Agar plate with 200 ul A6
- X-GAl plate with 20 ul of BBa_E0240 from glycerol stock (negative control).
Expected results:
- Plates 1 and 2 should develop blue colonies
- Plate 4 should remain colorless
16 hr incubation at 37°C.
June, 18th
June, 19th
June, 20th
June, 21st
"
