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Team:UCSF/Notebook
From 2009.igem.org
HL60 Group
- Main Goals:
- Engineering Navigation:
- sending cells to new targets - GPCR Screening
- tuning receptor sensitivity - Forced localization of effector proteins to GPCRs
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Dictyostelium Group
- Main Goals:
Engineering brakes and accelerators:
- modulating PIP3 polarity by building synthetic protein based feedback loops
workflow:
- 1. PCR parts: catalytic and localization domains
- 2. Create combinations of localization domains and catalytic domains
- 3. Generate Dictyostelium strains expressing feedback elements
- 4. Measure motility parameters (speed, directionality)
- 3. Analyze data. Create histograms and compare if our plasmid had any effect to dicty.Do they cause them to move faster or slower?
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- Allen Cai's notebook:
- Alex Smith's notebook:
- Edna Miao's notebook: Transformed new constructs into Dicty, analyzed data, and worked with PTEN null dicty.
- Ethan Chan's notebook: Constructed Dicty cells with new parts, analyzed data, and worked with pTen mutated dicty cells.
- Ryan Quan's notebook:
