Digestion of the following Biobricks with the indicated enzimes:
R0079
Prefix
E, S
F1610
Sufix
E, X
B0034
C0078
C0079
B0015
Cuts were done thinking on the standard assembling 10 with the 2-way-ligation.
Procedement:
- Take the purified DNA stocks from the previous Biobricks - Take the enzimes and add 100 μl of Buffer
2 μl of each sample were analized on a Spectrophotometer to determine the concentration of DNA on each Biopart.
Biobrick
Concentration (ng/μl)
158.9
11.5
7.5
11.6
7.7
B0079
11.2
...
"
