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User contributions
From 2009.igem.org
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- 14:04, 20 July 2009 (diff | hist) N Team:Gaston Day School/7 July 2009 (New page: <html> <du> <dt>Spin 1.5mL of overnight culture for 30 sec (5') in microfuge</dt> <dt>Aspriate oft all bu 100h of the supernatant and resuspend the pellet by vortexing</dt> <dt>Add 30x of ...)
- 13:59, 20 July 2009 (diff | hist) N Team:Gaston Day School/6 July 2009 (New page: <html> <du> <dt>750x LB broth</dt> <dt>8 tubes</dt> <dt>2 of each</dt> <dt>Berkeley plasmid, cRFP, RFP, Stop</dt> <dt>Did not change tips when swticghting from kan and amp broths</dt> <dt>...) (top)
- 13:56, 20 July 2009 (diff | hist) N Team:Gaston Day School/24 June 2009 (New page: <html> <du> <dt>4 tubes each for cRFP, RFP, Stop, Narg</dt> <dt>750microliters of Broth to each</dt> <dt>Streak DNA into tubes</dt> <dt>Incubate</dt>) (top)
- 13:55, 20 July 2009 (diff | hist) N Team:Gaston Day School/23 June 2009 (New page: <html> <du> <dt>Plated comp. cells</dt> <dt>Plated 100microliters of cRFP and RFP</dt> <dt>Make gel w/ Carolina Blue dye</dt> <dt>Ladder, Uncut, Eco, Xba, Spe, Pst</dt>) (top)
- 13:53, 20 July 2009 (diff | hist) Team:Gaston Day School/22 June 2009 (top)
- 13:52, 20 July 2009 (diff | hist) Team:Gaston Day School/22 June 2009
- 14:56, 13 July 2009 (diff | hist) N Team:Gaston Day School/22 June 2009 (New page: <html> <du> <dt>High eff. EcoRI: RFP, constit. RFP</dt> <dt>Thaw e. Coli till ice disappears</dt> <dt>Pipette 100x into tube (X2)</dt> <dt>Add 10x of plasmid to e. Coli</dt> <dt>Place on i...)
- 14:28, 13 July 2009 (diff | hist) N Team:Gaston Day School/19 June 2009 (New page: <html> <du> <dt>High eff. e. Coli for (RFP, stop codon, constit. RFP)</dt> <dt>Thaw e. Coli till ice disappears</dt> <dt>Pippete 50x into tube X3</dt> <dt>Add and mix plasmid X3</dt> <dt>S...)
- 14:20, 13 July 2009 (diff | hist) Team:Gaston Day School/18 June 2009 (top)
- 14:18, 13 July 2009 (diff | hist) N Team:Gaston Day School/18 June 2009 (New page: <html> <du> <dt>Made 5 normal augar plates and 18 ampicillen plates</dt> <dt>Add 750microliters LB/Kan</dt> <dt>Oxygenate every 30 mins after incubation</dt> <dt>Streak 8 Colonies</dt> <dt...)
- 14:16, 13 July 2009 (diff | hist) N Team:Gaston Day School/17 June 2009 (New page: <html> <du> <dt>High Efficiency E. Coli</dt> <dt>Thaw tube of NEB 10-beta Comp. E. Coli cells on ice until ice disappears. Gently mix and pipette 50mL into transformation tube on ice.</dt>...)
- 14:09, 13 July 2009 (diff | hist) N Team:Gaston Day School/16 June 2009 (New page: <html> <du> <dt>Spin tubes #1-8 for 3 minutes</dt> <dt>Combined tubes #1-4 and tubes #5-8</dt> <dt>Added 300x TENS</dt> <dt>Mix by inversion</dt> <dt>Added 150x sodium acetate</dt> <dt>Mix...) (top)
- 14:06, 13 July 2009 (diff | hist) N Team:Gaston Day School/15 June 2009 (New page: <html> <du> <dt>Digest w/ EcoRI</dt> <dt>20x DNA</dt> <dt>3x Buffer</dt> <dt>5x dH2O</dt> <dt>2x EcoRI</dt> <dt>10:31AM</dt> <dt>Gel Electrophoresis</dt> <dt>Fast blast vs. Carolina blue</...) (top)
- 14:04, 13 July 2009 (diff | hist) N Team:Gaston Day School/12 June 2009 (New page: <html> <du> <dt>Spun tubes #1-8 for 3 minutes</dt> <dt>Combined tubes #1-4 and tubes #5-8</dt> <dt>Added 300x TENS</dt> <dt>Mix by inversion</dt> <dt>Added 150x sodium acetate</dt> <dt>Mix...) (top)
- 14:00, 13 July 2009 (diff | hist) N Team:Gaston Day School/10 June 2009 (New page: <html> <du> <dt>Digest w/ EcoRI</dt> <dt>10x DNA</dt> <dt>4x Buffer</dt> <dt>dH2O 24x</dt> <dt>2x EcoRI</dt> <dt>37C in</dt> <dt>New set #1-8</dt> <dt>LB 1mL kan</dt> <dt>Add 750x to each ...) (top)
- 15:59, 10 July 2009 (diff | hist) m Template:Team:Gaston Day School/Templates/Header
- 15:47, 10 July 2009 (diff | hist) N Team:Gaston Day School/9 June 2009 (New page: <html> <du> <dt>Resuspend in 30x TE</dt> <dt>Digest w/ EcoRI</dt> <dt>5x DNA</dt> <dt>2x Buffer</dt> <dt>11x dH20</dt> <dt>1x EcoRI</dt> <dt>37C in 10:45AM -- out 12:20PM</dt> <dt>Pour 2 1...) (top)
- 15:45, 10 July 2009 (diff | hist) N Team:Gaston Day School/8 June 2009 (New page: <html> <du> <dt>Tubes #5-8:</dt> <dt>Add 1 mL of room temp 70% Isopropynol</dt> <dt>Vortex DNA for 5 mins.</dt> <dt>Refrig.</dt> <dt>Dry o/n</dt>) (top)
- 15:44, 10 July 2009 (diff | hist) N Team:Gaston Day School/14 May 2009 (New page: <html> <du> <dt>Add 1mL EtOH to tubes 1-4</dt> <dt>Spin ~30-40 min - dump EtOH leave o/n 4C</dt> <dt>Add 300x TENS, 150x NaOAc to tubes 5-8</dt> <dt>Spin 20 min.</dt> <dt>Transfer sup to n...) (top)
- 15:42, 10 July 2009 (diff | hist) Team:Gaston Day School/13 May 2009 (top)
- 15:42, 10 July 2009 (diff | hist) N Team:Gaston Day School/13 May 2009 (New page: <html> <du> <dt>Tubes #1-4:</dt> <dt>Added 200microliters of TENS and mix</dt> <dt>Added 150microliters of sodium acetate and mix</dt> <dt>Centrifuged for 15-20 mins.</dt> <dt>Tubes #5-8:<...)
- 15:39, 10 July 2009 (diff | hist) N Team:Gaston Day School/12 May 2009 (New page: <html> <du> <dt>All 8 grew</dt> <dt>Spun 1.5 mL of overnight culture for 5 minutes in microfuge</dt> <dt>#1-4 left 5-8 o/n 37C</dt> <dt>Aspirated off all but 100microliters of the supernat...) (top)
- 15:33, 10 July 2009 (diff | hist) N Team:Gaston Day School/11 May 2009 (New page: <html> <du> <dt>LB+.5mL Kan</dt> <dt>Add 750x to each 2mL tube</dt> <dt>Streak 1 plate & 1 tube for 8 colonies</dt> <dt>Grown o/n 37C</dt>) (top)
- 15:32, 10 July 2009 (diff | hist) N Team:Gaston Day School/7 May 2009 (New page: <html> <du> <dt>All plates had lots of colonies</dt>) (top)
- 15:31, 10 July 2009 (diff | hist) N Team:Gaston Day School/6 May 2009 (New page: <html> <dl> <dt>Transform pNICE ~ E. coli/dt> <dt>50x competent cells on ice (Carolina Biol.)</dt> <dt>200x cold 50mM CaCl2</dt> <dt>10x 50ml pNICE (Lindow lab)</dt> <dt>Ice 15' 8:11--></d...) (top)
- 15:16, 10 July 2009 (diff | hist) N Team:Gaston Day School/4 May 2009 (New page: <html> <dl> <dt>Melt dB agar</dt> <dt>Add 4mL 10% Kanamycin (Carolina Biological)</dt> <dt>Pour Kan plates & mark w/ 1 black Line</dt>) (top)
- 15:01, 10 July 2009 (diff | hist) Team:Gaston Day School/Notebook (top)
- 14:59, 10 July 2009 (diff | hist) Team:Gaston Day School/Notebook
- 14:53, 10 July 2009 (diff | hist) Team:Gaston Day School/Notebook
- 14:48, 10 July 2009 (diff | hist) Template:Team:Gaston Day School/Templates/Header
- 14:21, 10 July 2009 (diff | hist) Template:Team:Gaston Day School/Templates/Header
- 14:12, 10 July 2009 (diff | hist) N File:Dropdowntabs.zip (top)
- 15:08, 9 July 2009 (diff | hist) Template:Team:Gaston Day School/Templates/Header
- 15:06, 9 July 2009 (diff | hist) Team:Gaston Day School/Parts
- 15:06, 9 July 2009 (diff | hist) Team:Gaston Day School/Project
- 15:05, 9 July 2009 (diff | hist) Team:Gaston Day School/Team
- 15:05, 9 July 2009 (diff | hist) Template:Team:Gaston Day School/Templates/Header
- 14:58, 9 July 2009 (diff | hist) Template:Team:Gaston Day School/Templates/Header
- 14:58, 9 July 2009 (diff | hist) Template:Team:Gaston Day School/Templates/Header
- 14:58, 9 July 2009 (diff | hist) Template:Team:Gaston Day School/Templates/Header
- 14:57, 9 July 2009 (diff | hist) Template:Team:Gaston Day School/Templates/Header
- 14:57, 9 July 2009 (diff | hist) Template:Team:Gaston Day School/Templates/Header
- 14:55, 9 July 2009 (diff | hist) Template:Team:Gaston Day School/Templates/Header
- 14:53, 9 July 2009 (diff | hist) Template:Team:Gaston Day School/Templates/Header
- 14:48, 9 July 2009 (diff | hist) Template:Team:Gaston Day School/Templates/Header
- 14:48, 9 July 2009 (diff | hist) Template:Team:Gaston Day School/Templates/Header
- 14:47, 9 July 2009 (diff | hist) Template:Team:Gaston Day School/Templates/Header
- 14:09, 9 July 2009 (diff | hist) Template:Team:Gaston Day School/Templates/Header (Removing all content from page)
- 14:53, 8 July 2009 (diff | hist) Template:Team:Gaston Day School/Templates/Header
- 14:51, 8 July 2009 (diff | hist) Template:Team:Gaston Day School/Templates/Header
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