Biobrick/
Ligation Name |
Description |
Creation Date |
Strain |
Vector |
Quality Control |
J23100(J100)
replaced by "J23100 Jun4" |
constitutive promoter |
May 25 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23101(J101)
replaced by "J23101 Jun4" |
constitutive promoter |
May 25 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23102(J102) |
constitutive promoter |
May 25 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23103(J103) |
constitutive promoter |
May 25 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23104(J104) |
constitutive promoter |
May 25 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23105(J105) |
constitutive promoter |
May 25 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23106(J106) |
constitutive promoter |
May 25 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23110(J110) |
constitutive promoter |
May 25 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23112(J112) |
constitutive promoter |
May 25 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23113(J113) |
constitutive promoter |
May 25 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23114(J114) |
constitutive promoter |
May 25 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23116(J116) |
constitutive promoter |
May 25 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23117(J117) |
constitutive promoter |
May 25 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23118(J118)
replaced by "J23118 Jun4" |
constitutive promoter |
May 25 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| E0240(GFP3) |
GFP protein generator
(RBS-GFP-TT) |
May 25 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence ok (MIT) |
| K131009(E2) |
colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS) |
May 28 |
TOP10 |
pSB1AK3 |
sequence BAD in prefix,suffix,celB (MIT,BMR) |
| K117000(celB) |
colE7 lysis gene |
May 28 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
| K124017(lysis21) |
Phage Lysis Cassette |
May 28 |
TOP10 |
pSB1AK3 |
sequence BAD (MIT) |
| B0015(TT) |
Double terminator |
May 28 |
TOP10 |
pSB1AK3 |
sequence ok (MIT) |
| I732017 (RBS-lacZ) |
RBS-lacZ |
May 28 |
TOP10 |
pSB1A2 |
long part (MIT) |
| B0030 |
RBS 0.6 |
Jun 4 |
TOP10 |
pSB1A2 |
sequence ok (MIT,BMR) |
| B0031 |
RBS 0.07 |
Jun 4 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
| B0032 |
RBS 0.3 |
Jun 4 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
| B0033 |
RBS 0.01 |
Jun 4 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
| B0034 |
RBS 1 |
Jun 4 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
| P0440(P04) |
RBS-tetR-TT |
Jun 4 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence ok (MIT) |
| R0040(Ptet) |
Ptet |
Jun 4 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
| T9002 |
Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT |
Jun 4 |
TOP10 |
pSB1A3 |
sequence BAD (MIT) - sequence ok (BMR) |
| J23100(J100 Jun4) |
constitutive promoter |
Jun 4 |
TOP10 |
J61002(Amp) |
sequence ok (MIT,BMR) |
| J23101(J101 Jun4) |
constitutive promoter |
Jun 4 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23118(J118 Jun4) |
constitutive promoter |
Jun 4 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| A1 |
J23100(Jun 4)-E0240
(Pcon-RBS-GFP-TT) |
Jun 11 |
TOP10 |
pSB1A2 |
length ok (PCR) |
| A2 |
J23101(Jun 4)-E0240
(Pcon-RBS-GFP-TT) |
Jun 11 |
TOP10 |
pSB1A2 |
length ok (PCR) |
| A3-1 |
I732017-B0015
(RBS-lacZ-TT) |
Jun 12 |
TOP10 |
pSB1AK3 |
length ok (digestion PstI) sequence ok -long part- (BMR) |
| A4 |
P0440-R0040
(RBS-tetR-TT-Ptet) |
Jun 11 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence ok (BMR) |
| A5 |
A4-A3
(RBS-tetR-TT-Ptet-RBS-lacZ-TT) |
Jun 19 |
TOP10 |
pSB1AK3 |
sequence ok -long part- (BMR) |
| A6 |
J23100(Jun 4)-A4
(Pcon-RBS-tetR-TT-Ptet) |
Jun 19 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence ok (BMR) |
| BOL1 |
J23118-B0034-C0012-B0015
(Pcon-RBS-lacI-TT) |
Jun 23 |
TOP10 |
pSB1A2 |
sequence ok (BMR) |
| A7 |
J23118(Jun 4)-E0240
(Pcon-RBS-GFP-TT) |
Jun 25 |
TOP10 |
pSB1A2 |
length ok (PCR) |
| R0011(Plac) |
Plac |
Jun 25 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
| K112808(ENTERO) |
holin-lysozyme-T-Pconweak-antiholin-T (with RBSs) |
Jun 25 |
TOP10 |
pSB1A2 |
sequence almost ok -long part- (MIT): it has several gaps in the scars, a silent point mutation in holin gene and a wrong annotated terminator at the end of the brick (that is actually K112710, not B0010). |
| A8 |
A4-E0240
(RBS-tetR-TT-Ptet-RBS-GFP-TT) |
Jun 29 |
TOP10 |
pSB1A2 |
NON PURE PLASMID! (digestion E-P) but works |
| A9 |
A6-E0240
(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT) |
Jun 29 |
TOP10 |
pSB1A2 |
NON PURE PLASMID! (digestion E-P) but works |
A10
thrown away |
K117000-B0015
(celB-TT) |
Jun 30 |
TOP10 |
pSB1AK3 |
sequence BAD (BMR) |
| A8pg |
A4-E0240
(RBS-tetR-TT-Ptet-RBS-GFP-TT) |
Jul 3 |
TOP10 |
pSB1A2 |
length ok (digestion E-P) |
| A9pg |
A6-E0240
(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT) |
Jul 3 |
TOP10 |
pSB1A2 |
length ok (digestion E-P) |
A11-1
thrown away and replaced |
BOL1-R0011
(Pcon-RBS-lacI-TT-Plac) |
Jul 10 |
TOP10 |
pSB1A2 |
length ok (PCR)
sequence MUTATED |
A11-3
thrown away and replaced |
BOL1-R0011
(Pcon-RBS-lacI-TT-Plac) |
Jul 10 |
TOP10 |
pSB1A2 |
length ok (PCR) |
A11-6
thrown away and replaced |
BOL1-R0011
(Pcon-RBS-lacI-TT-Plac) |
Jul 10 |
TOP10 |
pSB1A2 |
length ok (PCR) |
F2620
thrown away |
Ptet-RBS-luxR-TT-Plux
|
Jul 10 |
TOP10 |
pSB1A2 |
sequence BAD (MIT, BMR) |
| A12-2 |
J23118(Jun 4)-A4
|
Jul 16 |
TOP10 |
pSB1A2 |
length ok (PCR)
sequence ok (BMR) |
A12-3
it is a backup of A12-2 |
J23118(Jun 4)-A4
|
Jul 16 |
TOP10 |
pSB1A2 |
length ok (PCR)
sequence ok (BMR) |
A14-4
thrown away |
A11-E0240
|
Jul 16 |
TOP10 |
pSB1A2 |
NON PURE PLASMID! (digestion) and the A11 sample used for ligation was MUTATED (BMR) |
A14pg
thrown away |
A11-E0240
|
Jul 16 |
TOP10 |
pSB1A2 |
NON PURE PLASMID! (digestion) and the A11 sample used for ligation was MUTATED (BMR) |
| K116001 (PnhaA) |
PnhaA-RBS-GFP-TT
|
Jul 29 |
? |
pSB1A2 |
sequence WRONG! it is actually K116002!!! |
| K116002 (PnhaA+GFP) |
PnhaA-RBS-GFP-TT
|
Jul 29 |
? |
pSB1A2 |
sequence WRONG! it is actually J33204!!! |
| K112405 (Pcad) |
cadAB promoter
|
Jul 29 |
NEB 10-beta |
K112951(Amp+Cm) |
sequence ok (BMR) |
| P0412 |
RBS-lacI-TT
|
Jul 29 |
DH5alpha |
pSB1A2 |
sequence ok (BMR) |
| I746902 |
GFPmut3+his tag generator under Pbad
|
Jul 29 |
NEB 10-beta |
pSB1AK3 |
sequence ok -long part- (BMR) |
| I746903 |
GFPmut3+his tag generator under PT7
|
Jul 29 |
NEB 10-beta |
pSB1AK3 |
sequence almost ok (BMR): the RBS is B0030 and not B0034 as documented, but the function is the same |
K101017
to throw away! |
MioC promoter
|
Jul 29 |
NEB 10-beta |
pSB1A2 |
sequence BAD (BMR) |
| F2620MIT1 |
Ptet-RBS-luxR-TT-Plux
|
Jul 29 |
D1210(lacIq) |
pSB1A2 |
sequence ok (BMR) |
| F2620MIT2 |
Ptet-RBS-luxR-TT-Plux
|
Jul 29 |
D1210(lacIq) |
pSB1A2 |
sequence ok (BMR) |
| MRGENE1 |
pdc |
Jul 17 |
TOP10 |
pMK-RQ
(Kan) |
antibiotic ok
length ok (digestion X-P)
sequence ok (Mr Gene) |
| MRGENE1-2 |
pdc |
Jul 17 |
TOP10 |
pMK-RQ
(Kan) |
antibiotic ok
sequence ok (Mr Gene) |
| MRGENE1-3 |
pdc |
Jul 17 |
TOP10 |
pMK-RQ
(Kan) |
antibiotic ok
sequence ok (Mr Gene) |
| MRGENE2 |
adhB |
Jul 17 |
TOP10 |
pMA
(Amp) |
antibiotic ok
length ok (digestion X-P)
sequence ok (Mr Gene) |
| MRGENE2-2 |
adhB |
Jul 17 |
TOP10 |
pMA
(Amp) |
antibiotic ok
sequence ok (Mr Gene) |
| MRGENE2-3 |
adhB |
Jul 17 |
TOP10 |
pMA
(Amp) |
antibiotic ok
sequence ok (Mr Gene) |
| B1-13 |
B0030-MRGENE1
(RBS-pdc) |
Jul 28 |
TOP10 |
pSB1A2 |
length ok (digestion E-P
, digestion S-P and digestion E-S)
sequence ok (BMR) |
| B2-5 |
B0030-MRGENE2
(RBS-adhB) |
Jul 23 |
TOP10 |
pSB1A2 |
length ok (PCR
and digestion S-P)
sequence ok (BMR) |
| B3-5 |
B1-B0015
(RBS-pdc-TT) |
Jul 31 |
TOP10 |
pSB1AK3 |
length ok+extra band (digestion XhoI-P
and digestion X-P)
sequence ok (BMR) |
| B4-2 |
B2-B0015
(RBS-adhB-TT) |
Jul 31 |
TOP10 |
pSB1AK3 |
length ok (digestion XhoI-P)
sequence ok (BMR) |
| B5-3 |
B1-B4
(RBS-pdc-RBS-adhB-TT) |
Aug 12 |
TOP10 |
pSB1AK3 |
length ok+2 extra bands (digestion E-P)
sequence ok (BMR) |
| B6-3 |
B2-B3
(RBS-adhB-RBS-pdc-TT) |
Aug 12 |
TOP10 |
pSB1AK3 |
length ok+2 extra bands (digestion E-P)
sequence ok (BMR) |
A11-2
thrown away |
BOL1-R0011
(Pcon-RBS-lacI-TT-Plac) |
Aug 13 |
TOP10 |
pSB1A2 |
length ok (digestion S-P) sequence MUTATED (BMR) |
| A11-3 |
BOL1-R0011
(Pcon-RBS-lacI-TT-Plac) |
Aug 13 |
TOP10 |
pSB1A2 |
length ok (digestion S-P) sequence ok (BMR) |
A15-1
it is a backup copy of A15-3 |
F2620MIT1-K112808
(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T) |
Aug 14 |
TOP10 |
pSB1A2 |
length ok (digestion) sequence ok (BMR) |
A15-2
thrown away |
F2620MIT1-K112808
(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T) |
Aug 14 |
TOP10 |
pSB1A2 |
length ok (digestion) sequence WRONG (BMR) |
| A15-3 |
F2620MIT1-K112808
(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T) |
Aug 14 |
TOP10 |
pSB1A2 |
length ok (digestion) sequence ok (BMR) |
B8-5
thrown away |
A4-B6
(RBS-tetR-TT-Ptet-RBS-adhB-RBS-pdc-TT) |
Aug 21 |
TOP10 |
pSB1AK3 |
length ??? (digestion E-P) sequence WRONG (BMR) |
A14-3
to throw away! |
A11(-2 Aug13)-E0240
(Pcon-RBS-lacI-TT-Plac-RBS-GFP-TT) |
Aug 21 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence of A11-2(Aug13) was MUTATED (BMR) |
| A16-4 |
A12-E0240
(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT) |
Aug 21 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence ok (BMR) |
A17-4
to throw away! |
R0011-E0240
(Plac-RBS-GFP-TT) |
Aug 28 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence WRONG! ligation failed. |
A18
thrown away |
A15(-2)-pSB3K3
(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T) |
Aug 28 |
TOP10 |
pSB3K3 |
not screened, its parent plasmid (A15-2) was wrong |
| K116002 (PnhaA+GFP) |
PnhaA-RBS-GFP-TT
|
Sep 4 |
TOP10 |
pSB1A2 |
sequence almost ok (BMR): inconsistent prefix (but restriction sites are good) and additional "c" at the end of promoter |
| B5new2-3 |
B1-B4 (RBS-pdc-RBS-adhB-TT) |
? |
TOP10 |
pSB1AK3 |
length ok (digestion E-P), sequence ok -long part- (BMR) |
| B9-2 |
F2620-B5 (Ptet-RBS-luxR-TT-Plux-RBS-pdc-RBS-adhB-TT) |
? |
TOP10 |
pSB1AK3 |
length ok (digestion E-P), sequence ok -long part- (BMR) |
| B10-2 |
F2620-B6 (Ptet-RBS-luxR-TT-Plux-RBS-adhB-RBS-pdc-TT) |
? |
TOP10 |
pSB1AK3 |
length ok + 2 extra bands (digestion E-P), sequence ok -long part- (BMR) |
| B9new-1 to 5 |
F2620-B5new2(-3) (Ptet-RBS-luxR-TT-Plux-RBS-pdc-RBS-adhB-TT) |
? |
TOP10 |
pSB1AK3 |
length ok (digestion E-P) |
| A19 |
F2620 |
? |
TOP10 |
pSB4C5 |
length ok (digestion E-P) |
| A21 |
A19-B5new2(-3) |
? |
TOP10 |
pSB4C5 |
length ok (digestion E-P) |
| A14S-3 |
J23118-RBS-lacI-TT-Plac-RBS-GFP-TT (A11(-3)-E0240) |
? |
TOP10 |
pSB1A2 |
length ok (digestion E-P) |
| A17new |
Plac-RBS-GFP-TT (R0011-E0240) |
? |
TOP10 |
pSB1A2 |
length ok (PCR), sequence ok (BMR) |
| A20 |
F2620-A8 (Ptet-RBS-luxR-TT-Plux-RBS-tetR-TT-Ptet-RBS-GFP-TT) |
? |
TOP10 |
pSB1A2 |
length ok (digestion E-P) |
| I52002 (4C5) |
plasmid |
? |
TOP10 |
pSB4C5 |
length ok (digestion E-P) |
| I714891 (3K3) |
plasmid |
? |
TOP10 |
pSB3K3 |
length ok (digestion E-P) |
| B14 |
F2620-B3 (Ptet-RBS-luxR-TT-Plux-RBS-pdc-TT) |
? |
TOP10 |
pSB1A2 |
to screen |
| B15 |
F2620-B4 (Ptet-RBS-luxR-TT-Plux-RBS-adhB-TT) |
? |
TOP10 |
pSB1A2 |
to screen |
"
