HKU-HKBU/29 July 2009

From 2009.igem.org

Revision as of 14:30, 16 October 2009 by Paultse (Talk | contribs)

Co-transformation Transform two plasmids (A+, C+) to MG3.① Plac-CheZ-His-TT C+② Lac Iq A+

14:10 Subculture to warm room (37℃), shaking for 1.5h.
15:40 take the subculture bacteria out from the warm room.Freeze the bacteria in 0℃ ice water for
15min.Centrifuge in 4000rmp at 0℃ for 2min.Decent supernatant
Retrieved from "http://2009.igem.org/HKU-HKBU/29_July_2009"