Team:HKUST/Result3

Welcome

Constructs

We have successfully cloned the FUS1t gene into the multiple cloning site of the plasmid pRS426. The agarose gel electrophoresis pictures showing the correct sizes after enzyme digestion of pRS426-tFUS1 is shown in Figure 6. The other parts, the FUS1 promoter and the EGFP gene are still under construction. Also, the ARO9 gene has been amplified from yeast genome.

Figure 6 agarose gel electrophoresis pictures showing the correct sizes after enzyme digestion of pRS426-tFUS1

Background

Experimental design

Parts design

Experimental result

Future work

Reference