Team:Freiburg bioware/cloning1

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Index

*His+FluA+Split+Foki_pMA

* His+FluA+longlinker+Foki_pMA

 Parts

 Strep+FluA+shortlinker+FokA_pMA

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Strep+FluA+shortlinker+FokA in pMA Vector

nucleotid sequence of ORF-1: ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAG GAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTA TATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGG AAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAA CATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTC CCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTT ATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAG AACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTA TCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTA AAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGC AATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGAT CAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATG GGGAGATCAATTTTACCGGTTAA

AA sequence of ORF-1:

MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEF FMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKE NQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEEL LIGGEMIKAGTLTLEEVRRKFNNGEINFTG

Commented GenBank file

Strep+FluA+shortlinker+FokA_pMA.txt

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