Team:Wisconsin-Madison/Notebook Protocols

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Protocols

Plasmid Preparation by Alkaline Lysis
Plasmid Preparation by Boiling Lysis
Restriction Digestion
Transformation of Plasmids into Competant Cells
Preparation of Competent Cells
Cloning Protocols
Polymerase Chain Reaction (PCR)
Ligation
Digestion - Cloning
Inoculation of Plasmids in Liquid Media
Inoculation of Cyanobacteria
Loading Gel Protocol
Plasmid Clean-up
Gel Extraction from Agar
Sequencing Reaction
Colony Polymerase Chain Reaction (PCR)
Genomic DNA Extraction

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