Lab Oct 13 2009

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11am

Epi's put in for autoclaving, I'll be back at 12.30pm to do minipreps and likely a gel, possibly assemblies.

1.30pm

Minipreps of K235030 Kan, K235030 Chl, and K235031 Chl started.

Gel for K235030 Kan, K235030 Chl, K235031 Chl, E0840 Amp, and K115020 Amp started at 3.30pm

Oct 13 gel 1.tif Ladder K115020 E0840 K235030 Kan K235030 Chl K235031

 	Band 	Band 	Band 	Band 	Band

Bands for everything! I'm going ahead with the next assemblies: K235032 Kan, 33 Kan, 36 Kan, and 37 Chl.

I will also transform new cultures of J04630 Kan and K235005 Chl to use as controls when testing for flouresence.

10pm

K235032 Kan, 33 Kan, 36 Kan, 37 Chl, J04630 Kan and K235005 Chl plated. I will be in tomorrow to do broth cultures at 10.30am.