Team:LCG-UNAM-Mexico:WTM

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WILD-TYPE MOLECULAR MODEL

Simbiology ® diagram of WTM

WTM simulate crucial reactions and events in the life cycle of phage T7:


  • Insertion and translocation of phage's DNA at different times


Phage's DNA is arranged in three classes of genes depending on their positions on the genome, T7 genome is translocated into the cell between 6 to 10 minutes after attachment, so this order and timing drives the phage's development.


  • Trancription of different T7 DNA segments into polycistronic mRNAs


Transcription of T7 polycistronic mRNAs occurs only if its coding DNA segment is available in the cell, for example early phage mRNAs are present before late mRNAs given that DNA responsible for them is inserted at early stages.

For early phage genes which are transcribed by bacterial RNA polymerase, rate limiting steps are ribosome elongation rate and length of polycistronic mRNA.

For phage genes with T7 RNA polymerase promoter rate limiting steps are abundance of T7 RNA polymerase, ribosome elongation rate and length of polycistronic mRNA.


  • Degradation of phage's mRNAs.


We assume that degradation rates for all polycistronic mRNA are the same as E.Coli



  • Translation of phage's mRNAs into proteins



  • T7 DNA replication
  • Procapsid Assembly
  • DNA packaging and final assembly
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