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From 2009.igem.org

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	LB medium DNA resuspension from iGEM 2009 plates Transformation X-Gal staining protocol for beta galactosidase (blue/white screening) Glycerol stocks Plasmid extraction BioBrick digestion with restriction enzymes Electrophoresis DNA gel extraction DNA precipitation with sodium acetate Ligation PCR Sensing ethanol concentration: our do-it-yourself kit

Gycerol stocks

(estimated time: 15 min + freezing time)

Materials needed:

• sample of overnight incubated bacteria
• 80% glycerol

• Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol
• Leave at -20°C for one day
• Move to -80°C the day after

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