Purification of pTet and double terminator biobricks
Once we digested those biobricks yesterday, we could proceed today to the purification of the target band from an agarose gel.
We used Invitrogen's Purelink Quick Gel Extraction Kit, following the manufacturer's protocol (Protocol 7), without modifications.
Marcelo
YeastGuard
Dephosphorylation - CIAP test
The E.coli didn´t grow in any of the plates again. =[ We discovered that the electrocompetent E.coli that we used yesterday were dead! So we transformed another electrocompetent E.coli using the rest of the ligation reactions we had. We extremely hope to find colonies tomorrow!
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