Team:Wisconsin-Madison/Polymerase Chain Reaction (PCR)

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Polymerase Chain Reaction (PCR)
  • To amplify and to add BioBrick Restriction sites to your DNA your run a PCR of your gene.
  • You must first design Primers for you gene.


Tips:

EcoR1 (E)

  • 5’- G A A T T G -3’
  • 3’- C T T A A G -5’

Xba1 (X)

  • 5’- T C T A G A -3’
  • 3’- A G A T C T -5’

Spec1 (S)

  • 5’- A C T A G T -3’
  • 3’- T G A T C A -5’

Pst1 (P)

  • 5’- C T G C A G -3’
  • 3’- G A C G T C -5’


X and S have same sticky ends

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