Team:Wisconsin-Madison/Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR)

• To amplify and to add BioBrick Restriction sites to your DNA your run a PCR of your gene.
• You must first design Primers for you gene.

Tips:

EcoR1 (E)

• 5’- G A A T T G -3’
• 3’- C T T A A G -5’

Xba1 (X)

• 5’- T C T A G A -3’
• 3’- A G A T C T -5’

Spec1 (S)

• 5’- A C T A G T -3’
• 3’- T G A T C A -5’

Pst1 (P)

• 5’- C T G C A G -3’
• 3’- G A C G T C -5’

X and S have same sticky ends