Team:Berkeley Wetlab/Recipes

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Contents

10X TBST

31.4 g TRIS HCl
80g NaCl
10ml Tween-20
bring volume to 1000ml
pH 7.5

1X TBS

605 mg Tris
876 mg NaCl
80 ml H2O
pH 7.4
bring volume to 100 ml

Artificial Seawater

500 ml ddH2O
12.2 g NaCl
2.6 g MgCl2
.57 g CaCl2

Tris Buffer + 2% SDS

50 mM Tris
150 mM NaCl
2% SDS
pH 7

Precipitation Buffer

2 mL of acetic acid
Add concentrated NaOH until the pH is 5
Add water to reach 10 mL total volume volume
0.2 g ZnCl
Add 40 mL ethanol


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