Team:Stanford/NotebookPage/6 August 2009

Daily Work

Goals

• Morning:
  • vBOL tutorial polishing
  • purchase omnigraffle
  • possible wiki template work
• Afternoon
  • Red papers for NO/SoxR affinity and Th-17 to NO pathway

Project / Accomplishments

• Long review of entire cloning process
• Learned to check sequences w/ Ape and order form (fwd and rev)
• Broke down project steps
• Finished new draft of presentation and went over it w/Jerome
  • Now contact physician and clinician
• Finished vBOL presentation
• Grew liquid cultures of B0015 and B0034, and part 53 and glycerol stock 9
• Started ligation for 1.1b (device one, first round of cloning, part b)
  • Right cycle on PCR?

Problems

• A little unsure about ligation protocol.... which PCR cycle? We chose ligation...

Alternative Solutions

Events

• 3pm meeting w/ Jerome about updated presentation... Feedback
  • More clear about age groups- is this when it starts?
  • Why industrial countries targeted? Sterile environments....
  • Where does it take place?
  • Genetic component of the disease?
  • Mouse model of IBD to test linear relationship of Th-17 and Treg cells
  • Colon slide- show parts w/ larger picture and then transition into video by itself
    • Naive cd4+ cells do not show up in the video on the screen
    • Positive feedback between t-cells and differentiation of naive cd4+ cells
  • Trp analog levels
  • Device one diagram: sensor will end at SoxS, then generator is entire sequence
  • Constitutive export system..
    • Constitutively expressing everything besides il-6
  • Missing consistent background of T-cells... larger description of immunology and mediated immune cells
  • Simple, fun animation... like the french cartoons
  • Take out th1 and th2 cells in picture
  • th-17 to t-reg cells stemming from Immune System
    • Go straight to the problem
  • Explain T-cells- what do they do? Like a detector....
    • Signal another cell to kill the pathogen...
    • talk about the recruitment of b-cells
  • Cartoon is not a system level diagram
  • New system level diagram- simple but w/ external features like b-cells and dendritic cells
  • Also, upstream of inflammation and immunosuppression symbols in diagram... disease...
  • Reuse presentation diagrams
  • Missing level between box pictures and basic parts diagram
    • Maybe boxes such as SoxR/S system= NO sensor
  • Introduce vBOL later, but not too much time
  • suicide device?
  • schematics w. numbers/letters
  • Other questions... do people take RA for treatment? Il-6? Methyl tryptophans for IDO
    • Check out other uses for the products

• From here
  • Contact clinician from here
  • Presentation works from here