| April
|
| M | T | W | T | F | S | S
|
|
|
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/1_April_2009&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_April_2009&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/3_April_2009&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/4_April_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/5_April_2009&action=edit 5]
|
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/6_April_2009&action=edit 6]
| [http://2009.igem.org/Wisconsin-Madison/7_April_2009 7]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/8_April_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_April_2009&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/10_April_2009&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_April_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/12_April_2009&action=edit 12]
|
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/13_April_2009&action=edit 13]
| [http://2009.igem.org/Wisconsin-Madison/14_April_2009 14]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/15_April_2009&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/16_April_2009&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/17_April_2009&action=edit 17]
| [http://2009.igem.org/Wisconsin-Madison/18_April_2009 18]
| [http://2009.igem.org/Wisconsin-Madison/19_April_2009 19]
|
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/20_April_2009&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_April_2009&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_April_2009&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_April_2009&action=edit 23]
| [http://2009.igem.org/Wisconsin-Madison/24_April_2009 24]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_April_2009&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_April_2009&action=edit 26]
|
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_April_2009&action=edit 27]
| [http://2009.igem.org/Wisconsin-Madison/28_April_2009 28]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_April_2009&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_April_2009&action=edit 30]
|
|
| May
|
| M | T | W | T | F | S | S
|
|
|
|
|
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/1_May_2009&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_May_2009&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/3_May_2009&action=edit 3]
|
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/4_May_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/5_May_2009&action=edit 5]
| [http://2009.igem.org/Wisconsin-Madison/6_May_2009 6]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/7_May_2009&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/8_May_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_May_2009&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/10_May_2009&action=edit 10]
|
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_May_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/12_May_2009&action=edit 12]
| [http://2009.igem.org/Wisconsin-Madison/13_May_2009 13]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/14_May_2009&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/15_May_2009&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/16_May_2009&action=edit 16]
| [http://2009.igem.org/Wisconsin-Madison/17_May_2009 17]
|
| [http://2009.igem.org/Wisconsin-Madison/18_May_2009 18]
| [http://2009.igem.org/Wisconsin-Madison/19_May_2009 19]
| [http://2009.igem.org/Wisconsin-Madison/20_May_2009 20]
| [http://2009.igem.org/Wisconsin-Madison/21_May_2009 21]
| [http://2009.igem.org/Wisconsin-Madison/22_May_2009 22]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_May_2009&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_May_2009&action=edit 24]
|
| [http://2009.igem.org/Wisconsin-Madison/25_May_2009 25]
| [http://2009.igem.org/Wisconsin-Madison/26_May_2009 26]
| [http://2009.igem.org/Wisconsin-Madison/27_May_2009 27]
| [http://2009.igem.org/Wisconsin-Madison/28_May_2009 28]
| [http://2009.igem.org/Wisconsin-Madison/29_May_2009 29]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_May_2009&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/31_May_2009&action=edit 31]
|
|
| June
|
| M | T | W | T | F | S | S
|
| [http://2009.igem.org/Wisconsin-Madison/1_June_2009 1]
| [http://2009.igem.org/Wisconsin-Madison/2_June_2009 2]
| [http://2009.igem.org/Wisconsin-Madison/3_June_2009 3]
| [http://2009.igem.org/Wisconsin-Madison/4_June_2009 4]
| [http://2009.igem.org/Wisconsin-Madison/5_June_2009 5]
| [http://2009.igem.org/Wisconsin-Madison/6_June_2009 6]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/7_June_2009&action=edit 7]
|
| [http://2009.igem.org/Wisconsin-Madison/8_June_2009 8]
| [http://2009.igem.org/Wisconsin-Madison/9_June_2009 9]
| [http://2009.igem.org/Wisconsin-Madison/10_June_2009 10]
| [http://2009.igem.org/Wisconsin-Madison/11_June_2009 11]
| [http://2009.igem.org/Wisconsin-Madison/12_June_2009 12]
| [http://2009.igem.org/Wisconsin-Madison/13_June_2009 13]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/14_June_2009&action=edit 14]
|
| [http://2009.igem.org/Wisconsin-Madison/15_June_2009 15]
| [http://2009.igem.org/Wisconsin-Madison/16_June_2009 16]
| [http://2009.igem.org/Wisconsin-Madison/17_June_2009 17]
| [http://2009.igem.org/Wisconsin-Madison/18_June_2009 18]
| [http://2009.igem.org/Wisconsin-Madison/19_June_2009 19]
| [http://2009.igem.org/Wisconsin-Madison/20_June_2009 20]
| [http://2009.igem.org/Wisconsin-Madison/21_June_2009 21]
|
| [http://2009.igem.org/Wisconsin-Madison/22_June_2009 22]
| [http://2009.igem.org/Wisconsin-Madison/23_June_2009 23]
| [http://2009.igem.org/Wisconsin-Madison/24_June_2009 24]
| [http://2009.igem.org/Wisconsin-Madison/25_June_2009 25]
| [http://2009.igem.org/Wisconsin-Madison/26_June_2009 26]
| [http://2009.igem.org/Wisconsin-Madison/27_June_2009 27]
| [http://2009.igem.org/Wisconsin-Madison/28_June_2009 28]
|
| [http://2009.igem.org/Wisconsin-Madison/29_June_2009 29]
| [http://2009.igem.org/Wisconsin-Madison/30_June_2009 30]
|
|
| July
|
| M | T | W | T | F | S | S
|
|
|
| [http://2009.igem.org/Wisconsin-Madison/1_July_2009 1]
| [http://2009.igem.org/Wisconsin-Madison/2_July_2009 2]
| [http://2009.igem.org/Wisconsin-Madison/3_July_2009 3]
| [http://2009.igem.org/Wisconsin-Madison/4_July_2009 4]
| [http://2009.igem.org/Wisconsin-Madison/5_July_2009 5]
|
| [http://2009.igem.org/Wisconsin-Madison/6_July_2009 6]
| [http://2009.igem.org/Wisconsin-Madison/7_July_2009 7]
| [http://2009.igem.org/Wisconsin-Madison/8_July_2009 8]
| [http://2009.igem.org/Wisconsin-Madison/9_July_2009 9]
| [http://2009.igem.org/Wisconsin-Madison/10_July_2009 10]
| [http://2009.igem.org/Wisconsin-Madison/11_July_2009 11]
| [http://2009.igem.org/Wisconsin-Madison/12_July_2009 12]
|
| [http://2009.igem.org/Wisconsin-Madison/13_July_2009 13]
| [http://2009.igem.org/Wisconsin-Madison/14_July_2009 14]
| [http://2009.igem.org/Wisconsin-Madison/15_July_2009 15]
| [http://2009.igem.org/Wisconsin-Madison/16_July_2009 16]
| [http://2009.igem.org/Wisconsin-Madison/17_July_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_July_2009&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/19_July_2009&action=edit 19]
|
| [http://2009.igem.org/Wisconsin-Madison/20_July_2009 20]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_July_2009&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_July_2009&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_July_2009&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_July_2009&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_July_2009&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_July_2009&action=edit 26]
|
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_July_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/28_July_2009&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_July_2009&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_July_2009&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/31_July_2009&action=edit 31]
|
|
| August
|
| M | T | W | T | F | S | S
|
|
|
|
|
|
| [http://2009.igem.org/Wisconsin-Madison/1_August_2009 1]
| [http://2009.igem.org/Wisconsin-Madison/2_August_2009 2]
|
| [http://2009.igem.org/Wisconsin-Madison/3_August_2009 3]
| [http://2009.igem.org/Wisconsin-Madison/4_August_2009 4]
| [http://2009.igem.org/Wisconsin-Madison/5_August_2009 5]
| [http://2009.igem.org/Wisconsin-Madison/6_August_2009 6]
| [http://2009.igem.org/Wisconsin-Madison/7_August_2009 7]
| [http://2009.igem.org/Wisconsin-Madison/8_August_2009 8]
| [http://2009.igem.org/Wisconsin-Madison/9_August_2009 9]
|
| [http://2009.igem.org/Wisconsin-Madison/10_August_2009 10]
| [http://2009.igem.org/Wisconsin-Madison/11_August_2009 11]
| [http://2009.igem.org/Wisconsin-Madison/12_August_2009 12]
| [http://2009.igem.org/Wisconsin-Madison/13_August_2009 13]
| [http://2009.igem.org/Wisconsin-Madison/14_August_2009 14]
| [http://2009.igem.org/Wisconsin-Madison/15_August_2009 15]
| [http://2009.igem.org/Wisconsin-Madison/16_August_2009 16]
|
| [http://2009.igem.org/Wisconsin-Madison/17_August_2009 17]
| [http://2009.igem.org/Wisconsin-Madison/18_August_2009 18]
| [http://2009.igem.org/Wisconsin-Madison/19_August_2009 19]
| [http://2009.igem.org/Wisconsin-Madison/20_August_2009 20]
| [http://2009.igem.org/Wisconsin-Madison/21_August_2009 21]
| [http://2009.igem.org/Wisconsin-Madison/22_August_2009 22]
| [http://2009.igem.org/Wisconsin-Madison/23_August_2009 23]
|
| [http://2009.igem.org/Wisconsin-Madison/24_August_2009 24]
| [http://2009.igem.org/Wisconsin-Madison/25_August_2009 25]
| [http://2009.igem.org/Wisconsin-Madison/26_August_2009 26]
| [http://2009.igem.org/Wisconsin-Madison/27_August_2009 27]
| [http://2009.igem.org/Wisconsin-Madison/28_August_2009 28]
| [http://2009.igem.org/Wisconsin-Madison/29_August_2009 29]
| [http://2009.igem.org/Wisconsin-Madison/30_August_2009 30]
|
| [http://2009.igem.org/Wisconsin-Madison/31_August_2009 31]
|
|
| September
|
| M | T | W | T | F | S | S
|
|
| [http://2009.igem.org/Wisconsin-Madison/1_September_2009 1]
| [http://2009.igem.org/Wisconsin-Madison/2_September_2009 2]
| [http://2009.igem.org/Wisconsin-Madison/3_September_2009 3]
| [http://2009.igem.org/Wisconsin-Madison/4_September_2009 4]
| [http://2009.igem.org/Wisconsin-Madison/5_September_2009 5]
| [http://2009.igem.org/Wisconsin-Madison/6_September_2009 6]
|
| [http://2009.igem.org/Wisconsin-Madison/7_September_2009 7]
| [http://2009.igem.org/Wisconsin-Madison/8_September_2009 8]
| [http://2009.igem.org/Wisconsin-Madison/9_September_2009 9]
| [http://2009.igem.org/Wisconsin-Madison/10_September_2009 10]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_September_2009&action=edit 11]
| [http://2009.igem.org/Wisconsin-Madison/12_September_2009 12]
| [http://2009.igem.org/Wisconsin-Madison/13_September_2009 13]
|
| [http://2009.igem.org/Wisconsin-Madison/14_September_2009 14]
| [http://2009.igem.org/Wisconsin-Madison/15_September_2009 15]
| [http://2009.igem.org/Wisconsin-Madison/16_September_2009 16]
| [http://2009.igem.org/Wisconsin-Madison/17_September_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_September_2009&action=edit 18]
| [http://2009.igem.org/Wisconsin-Madison/19_September_2009 19]
| [http://2009.igem.org/Wisconsin-Madison/20_September_2009 20]
|
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_September_2009&action=edit 21]
| [http://2009.igem.org/Wisconsin-Madison/22_September_2009 22]
| [http://2009.igem.org/Wisconsin-Madison/23_September_2009 23]
| [http://2009.igem.org/Wisconsin-Madison/24_September_2009 24]
| [http://2009.igem.org/Wisconsin-Madison/25_September_2009 25]
| [http://2009.igem.org/Wisconsin-Madison/26_September_2009 26]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_September_2009&action=edit 27]
|
| [http://2009.igem.org/Wisconsin-Madison/28_September_2009 28]
| [http://2009.igem.org/Wisconsin-Madison/29_September_2009 29]
| [http://2009.igem.org/Wisconsin-Madison/30_September_2009 30]
|
|
| October
|
| M | T | W | T | F | S | S
|
|
|
|
| [http://2009.igem.org/Wisconsin-Madison/1_October_2009 1]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_October_2009&action=edit 2]
| [http://2009.igem.org/Wisconsin-Madison/3_October_2009 3]
| [http://2009.igem.org/Wisconsin-Madison/4_October_2009 4]
|
| [http://2009.igem.org/Wisconsin-Madison/5_October_2009 5]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/6_October_2009&action=edit 6]
| [http://2009.igem.org/Wisconsin-Madison/7_October_2009 7]
| [http://2009.igem.org/Wisconsin-Madison/8_October_2009 8]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_October_2009&action=edit 9]
| [http://2009.igem.org/Wisconsin-Madison/10_October_2009 10]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_October_2009&action=edit 11]
|
| [http://2009.igem.org/Wisconsin-Madison/12_October_2009 12]
| [http://2009.igem.org/Wisconsin-Madison/13_October_2009 13]
| [http://2009.igem.org/Wisconsin-Madison/14_October_2009 14]
| [http://2009.igem.org/Wisconsin-Madison/15_October_2009 15]
| [http://2009.igem.org/Wisconsin-Madison/16_October_2009 16]
| [http://2009.igem.org/Wisconsin-Madison/17_October_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_October_2009&action=edit 18]
|
| [http://2009.igem.org/Wisconsin-Madison/19_October_2009 19]
| [http://2009.igem.org/Wisconsin-Madison/20_October_2009 20]
| [http://2009.igem.org/Wisconsin-Madison/21_October_2009 21]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_October_2009&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_October_2009&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_October_2009&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_October_2009&action=edit 25]
|
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_October_2009&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_October_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/28_October_2009&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_October_2009&action=edit 29]
| [http://2009.igem.org/Wisconsin-Madison/30_October_2009 30]
| [http://2009.igem.org/Wisconsin-Madison/31_October_2009 31]
|
|
June 15, 2009
Ex 13: Speced Choline, ProU, YhfR, NudF from PCR Product and DNA is in very high conc.
Ran Gel: Only have Primers UNSUCCESSFUL PCR (rerun in 2 different procedures)
4uL of NTP’s instead of 1uL
* PCR reaction tubes are in a draw on the island
Re-Run #1: Phussion – 1.5 hr 50uL rxn
1uL Forward Primer
1uL Reverse Primer
0.5uL DNA Template
0.5uL DNA Polymerase
1uL 10mM dNTPs (in box in -20 that looks like tip box – use one vial
10uL 5x Phusion HF Buffer
Re-Run #2: Taq – 2.5 hr. 50uL rxn
1uL Forward Primer
1uL Reverse Primer
2uL DNA Template
21uL water
25uL Taq Master Mix
Ran Gels: 2.0% Agarose, 100bp Ladder, 25min, 90V
Phus(ProU, YhfR, NudF, Choline) L, Taq(Choline, ProU, YhfR, NudF), Temp(ProU, YhfR, NudF, Choline), Blank(14,15)
Results: PCR2: taq and Phussion are successes, single bands of products.
Ex 9: miniprep of Choline gene ran gel
Ex 11: Check plate readings and they look good, almost done, will stop at end of day and compile data into growth curves
SEE: Salt Growth excel
NOTE: normal LB is made with 0.15 M Salt concentrations explaining the optimum grown at the 0.1 M test in salt-free LB
Ex12: Ethyl Acetate Extractions: ELABORATE
Span both of LB and Terrific Broth samples
Stored in -4 C fridge until we have GC time tomorrow night
Ex 10: Talked with Jon and Dan about lack of growth in 5:1 dilution cyano cultures, Probably due to bleaching of bad BG-11. Inoculated new 5:1 culture from joe’s stock and transferred cultures to plate (50uL 7942 and 1uL pmmBRraA) (50uL 7942 control on cm – BG11 plates). Waited 2hours for DNA to absorb then plated and put in light room
If works will give cyanobacteria chloramphenicol resistance
Attempted first natural transformation of PCC 7942 with pMMBRraA (50uL of culture and 1uL of DNA on BG11+CM plates, let sit 2 hours before plating) - NP
- ethyl acetate extraction for GC (3.5 mL induced culture and 1.75mL ethyl acetate, not GC grade, mixed for 5 mins, centrifuged at 2000 rpm for 10 mins then removed 450uL of supernatant ethyl acetate and put into GC vials) - NP
|
"
