Component | Part/Accession # | Base Pairs | Plasmid | Resistance | Well |
RBS | BBa_B0034 | 12 | pSB1A2 | A | plate 1, 2M |
Red Light Sensor | BBa_I15010 | 2,238 | pSB2K3 | K | separate |
OmpR (E. coli) | NP_417864.1 | 720 | pSB1T3 | T | separate |
Terminator | BBa_B0015 | 129 | pSB1AK3 | A and K | plate 1, 23L |
OmpR + Terminator | N/A | calculate | pany-amp | A | N/A |
OmpC promoter | BBa_R0082 | 108 | pSB1A2 | A | plate 1, 16K |
puc B/A | N/A | 375 | pSB1A3 | N/A |
Component | Part/Accession# | Base Pairs | Notes | Plasmid | Resistance | Well |
RBS | 12 | pSB1A2 | A | plate 1, 2M | ||||
Light Sensor | Light Sensor | 2,238 | pSB2K3 | K | separate | |||
OmpR (E. Coli) | NP_417864.1 | 720 | 3' --> 5' | pSB1T3 | T | separate | ||
Terminator | 129 | pSB1AK3 | AK | plate 1, 23L | ||||
OmpR + terminator | part | bp | pany-amp | A |
OmpC Promoter | 108 | pSB1A2 | A | plate 1, 16K | ||||
LH2 Output | pucB/A | R. Sphaeroides | 375 | (-40 of B to stop of A) | pSB1A3 | A | separate | |
A | YP_353390 | # | separate | |||||
B | YP_353391 | # | ||||||
OmpC promoter+BA | nopart number | bp | notes | pany-kana | K | not on plate |
PCB prod | Light Resp Sys | 4,333 | *Source of Hol/PcyA | plasmid | A | separate | ||
TetR repressible | 74 | notes | pSB1A2 | A | plate 1, 13B |
GFP Marker | Green Flourescence | 976 | notes | pSB1A2 | A | plate 1, 12M |