Team:Berkeley Wetlab/Cell Surface Display Parts

Follow any of the links below for more information about each of the passengers we made.

Streptavidin A tag that binds the protein streptavidin!	Leucine Zippers A structural motif that can allow different cell types to recognize and to bind each other!	Ag4 Peptide A peptide that reduces silver ions to form a silver precipitate!
MGFP-5 A protein used by mussels to stick to rocks. An underwater bio-glue!	Cellulases Enzymes that degrade cellulose!	TypeIII Needle scFv An antibody that binds a motif common to enteropathogenic bacteria!

• Displayers

Protein localization - The general scheme of protein localization is as shown in the following diagram.

Gram-negative bacteria have double membranes that present a problem for protein localization. Several protein transport mechanisms have been evolved to deal with this problem. These mechanisms include TypeI - TypeV secretion.

In our display systems, we used mainly autotransporters and some circularly permuted proteins. Autotransporters belong to a class of outermembrane proteins that is part of the TypeV secretion system common in many prokaryotic organisms.

azo1653 AtD OprF AtD Cl02365 AtD VtaA11 Hag AtD Pcryo_1225AtD Hia AtD upaG_short espP(beta) ehaB TshA VirG YuaQ AtD AIDA-I AtD Ag43_short

Repeated portion of ice nucleation protein (INP) sequence.

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