IBB Pune/6 October 2009

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We isolated the first set of ligations of

  • PR stands for Promoter-RBS BBa_J13002
  • Cprom stands for Constitutive promoter BBa_J23102

1) PLuxR-LuxI in pSB1Ak3 2) PR-GFP in pSB1Ak3 3) PLuxR-LuxI in pSB1A2 4) PR-GFP in pSB1A2

Assembly:

Digestion: 1) PR ES 2) Cprom ES 3) PluxR ES 4) LuxR SP 5) LacO SP 6) pSB1A2 EP

Ligations:

1) pLuxR-lacO-in pSB1A2 2) PR-LuxR in pSB1A2 3) Cprom-LuxR in pSB1A2

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