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Team:IIT Madras/Notebook/Sandbox
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Contents |
Intermediates and proposed constructs for the the 2 plasmid locking system
Original design
In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).
Final design
We decided to remove the extra antibiotic markers from the inserts. Then it looks like this
We had more than one strategy to build the whole construct.
strategy 1
strategy 2
Intermediates and proposed constructs for the the 3 plasmid locking system
We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too.
