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Team:IIT Madras/Notebook/Sandbox
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Contents |
Intermediates and proposed constructs for the the 2 plasmid locking system
Original design
In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).
Plasmid 1
Final design
We decided to remove the extra antibiotic markers from the inserts. Then it looks like this
We had more than one strategy to build the whole construct.
strategy 1
strategy 2
Intermediates and proposed constructs for the the 3 plasmid locking system
We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too.
