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Project Results

Our Results can be roughly divided in two sections, the biobrick assembly from starting parts in order to create our (test)-devices and secondly the characterizations of a part.

- First and foremost we succesfully assembled the following biobricks:

Partnumber Description

Antisense key

Sam5 + rbs

virA

VirG

VirB

Blue light promotor

Ech+fcs

Blp + GFP

Blp + RFP

EF + terminator

EF+ promoter+ terminator

Sam5 +sam8

Sams + promoter

Sams + promoter + comt

Sams+ comt+ promoter en terminator

lock

Sam8 + RBS

Ech + rbs

Fcs + rbs

All these parts were sent to the registry.

The blue light promotor was characterized, the strenght of the blue light promotor was compared to a normal promotor, furthermore the effect of promotor strenght on blue light irradiance was examined.

I20260} (orange) in RPU. The blue light promotor shows at least half the strenght of the standard promotor, meaning it can be classified as a weak promotor

Effect of blue light irradiance on promotor strenght. Cells grown in blue light irradiance (blue) show a stronger expression compared to cells grown in the dark (grey)