Team:Paris/Protocols

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Main - Microscope Protocol - Adapted Protocols - Culture protocols - Molecular Biology Main ===1. Microscopy=== * [[Team:Paris/ProtocolsA#Protocols suggested | Protocols suggested]] *[http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method (pdf)] ===2. Adapted Protocols=== * DNA extraction * [[Team:Paris/ProtocolsB#Adaptation of PureYield™ Plasmid Miniprep System (Promega) |Mini prep ]] * Gel/PCR Purification flefevre : si vous avez rien a dire ou pas le temps, il faut mieux supprimer des categories plutot que d avoir x pages pas créer ===3. Culture protocols=== * Over Night *[[Team:Paris/Protocols_Culture#Bacterial transformation | Bacterial transformation]] * [[Team:Paris/Protocols_Culture#Competent bacteria | Competent bacteria]] **[[team:Paris/Protocols_Culture#CaCl2a|CaCl₂ (iGEM Paris2007)]] **[[team:Paris/Protocols_Culture#CaCl2b|CaCl₂ (2nd Protocol)]] **[[team:Paris/Protocols_Culture#RbCl|RbCl]] * [[team:Paris/Protocols_Culture#Glycerol stock|Glycerol stock]] *[[team:Paris/Protocols_Culture#Ferric citrate growth medium|Ferric citrate growth medium]] ===4. Molecular biology=== *[[Team:Paris/ProtocolsMB#PCR with Quick load Taq2x Master Mix|PCR]] *[[Team:Paris/ProtocolsMB#PCR colonies|PCR colony]] * Gels * Digestion * Ligation