"
Team:HKUST/Protocols
From 2009.igem.org
a
- Agarose gel preparation and gel electrophoresis
- PCR (Taq, Vent)
- PCR product clean-up
- Enzyme digestion (vector, insert)
- Plasmid DNA extraction
- Gel cutting
- Gel extraction
- Ethanol precipitation
- Ligation
- Transformation to E.coli
- Transformation to yeast
- Cell lysis
- Yeast genomic DNA extraction
- Western blotting
- GFP testing
iGEM 2009
