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Team:Nevada/Team
From 2009.igem.org
Contents |
Who we are
Advisors
- Advisor 1: Dr. Christie Howard
- Advisor 2: Dr. Patricia Ellison
- Advisor 3: Dr. Dave Shintani
- Grad Student 1: Chad Sanada
Undergrads
- Student 1: Nick Tschernia
- Student 2: Tony Moschetti
- Student 3: Joey Khwaja
- Student 4: Chris Martinez
- Student 5: Janice Cho
- Student 6: Leigh Armijo
- Student 7: Sheena Jones
- Student 8: Tyler Peterson
- Student 9: J Malikowski
- Student 10: Alex Dussaq
What we did
Janice Cho and Leigh Armijo - Transformed and extracted DNA samples for lacI promoter, ribosome-binding site, double terminator, and various plasmid backbones. - Performed three-way ligation for the lacI promoter, ribsome-binding site, and a chloramphenicol resistant plasmid backbone. - Transformed mutated 4-coumarate:CoA ligase gene into DH5-alpha competent E. Coli cells.
J Malikowski
-Induced expression in non-biobrick expression vector containing 4-coumarate-CoA ligase (gene 2). Detection of product formation at 311 nm. Further studies will be conducted in the near future.
-Helped to troubleshoot minipreps and other protocols. Resident expert on minipreps.
-Trained Tyler to be an excellent Molecular Biologist.
-Advisory role at lab meetings (How does the Harper lab do it?)
Tyler Peterson -Major player in media preparation, agarose gels, stock solutions -Helped with minipreps and E. coli transformation.
Chris Martinez -Overall goal was to upregulate the cinnamaldehyde pathway in plants Wolffia and various Lemna species -Transformation of cinnamoyl-CoA Reductase (Gene 3) into TOPO D Vector -Used LR CLonase Protocal to move cinnamoyl-CoA Reductase (Gene 3) into pK7WG2D.1 Binary Vector which will be used to grow up Agrobacterium, which will be used to infect the plants Wolffia and Lemna species
Where we're from
