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Team:Nevada/Team
From 2009.igem.org
Contents |
Who we are
Advisors
- Advisor 1: Dr. Christie Howard
- Advisor 2: Dr. Patricia Ellison
- Advisor 3: Dr. Dave Shintani
- Grad Student 1: Chad Sanada
Undergrads
- Student 1: Nick Tschernia
- Student 2: Tony Moschetti
- Student 3: Joey Khwaja
- Student 4: Chris Martinez
- Student 5: Janice Cho
- Student 6: Leigh Armijo
- Student 7: Sheena Jones
- Student 8: Tyler Peterson
- Student 9: J Malikowski
- Student 10: Alex Dussaq
What we did
Janice Cho - Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000. - Helped conduct a three-way ligation to create final construct with the parts: BBa_K262000 and BBa_K262002.
Leigh Armijo - Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000. - Helped conduct a three-way ligation to create a construct with BBa_K262000, 4-coumarate:CoA ligase, and BBa_B0014.
Sheena Jones
-In charge of maintaining and propagating Wolffia and Lemna plant species.
-Generated callus culture in preparation for gene transformation.
Tony Moschetti
-Made Biobrick part containing cinnamoyl-CoA reductase (gene3) and double terminator (BBa_K262002)
-Transformed 4-coumarate-CoA ligase (gene 2) and cinnamoyl-CoA reductase (gene 3 - BBa_K262001) into Biobrick backbones.
J Malikowski
-Induced expression in non-biobrick expression vector containing 4-coumarate-CoA ligase (gene 2). Detection of product formation at 311 nm. Further studies will be conducted in the near future.
-Helped to troubleshoot minipreps and other protocols. Resident expert on minipreps.
-Trained Tyler to be an excellent Molecular Biologist.
-Advisory role at lab meetings (How does the Harper lab do it?)
Tyler Peterson
-Major player in media preparation, agarose gels, stock solutions
-Helped with minipreps and E. coli transformation.
Chris Martinez
-Overall goal was to upregulate the cinnamaldehyde pathway in plants Wolffia and various Lemna species
-Transformation of cinnamoyl-CoA Reductase (Gene 3) into TOPO D Vector
-Used LR CLonase Protocal to move cinnamoyl-CoA Reductase (Gene 3) into pK7WG2D.1 Binary Vector which will be used to grow up Agrobacterium, which will be used to infect the plants Wolffia and Lemna species
Where we're from
