Team:Alberta/Project/ISceIdigestion

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University of Alberta - BioBytes










































































































Linearizing Circularized Constructs by I-SceI Digestion

What you will need:

  • I-SceI
  • Biobyte Construct

Digestion:

For a 20 μL reaction:
  • 5 μL DNA sample
  • 2 μL NEB 10x I-SceI Buffer
  • 2 μL 10x BSA
  • 10.5 μL ddH2O
  • 0.5 μL I-SceI (5U/μL)

Incubate at 37ºC for 1 hour

Retrieved from "http://2009.igem.org/Team:Alberta/Project/ISceIdigestion"