From 2009.igem.org
Introduction
One of the famous black pigment is a Melanin which was utilized in a number of organism. Previously. It’s noted that the body color of E.coli was changed from white to black by a large amount of Melanin. Melanin was made by Tyrosinase (EC 1.14.18.1)(melA). Tyrosinase catalyze the first step of Melanin production pathway to make Dopaquinone. After production of Dopaquinone, Melanin is generated by nonenzymatical chain reaction. So, Overexprssion of tyrosinase in E.coli cause a large amount of Melanin. We considered these mechanism enable us to control the Mars temperature, get the heat energy that help to grow bacteria and, protect DNA from UV on the mars surface. Because of Melanin has a high efficiency heat absorption and absorb Ultra Violet.
What’s darker colored E.coli?
Why do we need darker colored E.coli?
Increase Mars temperature
Get the heat energy
Protect DNA
How to make darker colored E.coli
Utilize Melanin production pathway
Materials and Methods
Bacterial strains and cultivation
Construction of melA expressing vector
Analytical methods
Discussion
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