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From 2009.igem.org

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[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Tokyo_Tech Parts]

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Contents 1 Introduction 1.1 Increase Mars temperature 1.2 Get the heat energy 1.3 Protect DNA 2 How to make darker colored E.coli 2.1 Utilize Melanin production pathway 3 Materials and Methods 3.1 Bacterial strains and cultivation 3.2 Construction of melA expressing vector 3.3 Analytical methods 4 Discussion

Introduction

One of the famous black pigment is a Melanin which was utilized in a number of organism. Previously. It’s noted that the body color of E.coli was changed from white to black by a large amount of Melanin. Melanin was made by Tyrosinase (EC 1.14.18.1)(melA). Tyrosinase catalyze the first step of Melanin production pathway to make Dopaquinone. After production of Dopaquinone, Melanin is generated by nonenzymatical chain reaction. So, Overexprssion of tyrosinase in E.coli cause a large amount of Melanin. We considered these mechanism enable us to control the Mars temperature, get the heat energy that help to grow bacteria and, protect DNA from UV on the mars surface. Because of Melanin has a high efficiency heat absorption and absorb Ultra Violet.

Increase Mars temperature

Get the heat energy

Protect DNA

How to make darker colored E.coli

Utilize Melanin production pathway

Materials and Methods

Bacterial strains and cultivation

Construction of melA expressing vector

Analytical methods

Discussion

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