Team:HKUST/Protocols/gel cut

Cutting bands on the gel

Purpose: To extract specific DNA fragment.

Procedure:

a.Visualize the bands under UV light. Use long-wavelength UV to minimize damage to the DNA.
b.Cut the band with a clean razor blade.
c.Turn the gel slice on its side to trim off extra agarose. Place the gel in a microcentrifuge tube.

Tips:

EB staining is needed for higher resolution under UV light.

Safety tips:

1. ermission is needed from technician before doing this! Follow the safety instruction in the room.

2.When using UV light, protect your skin by wearing safety goggles or a face shield, gloves, and a lab coat.

• Agarose gel preparation and gel electrophoresis
• PCR (Taq, Vent)
• PCR product clean-up
• Enzyme digestion (vector, insert)
• Plasmid DNA extraction
• Gel cutting
• Gel extraction
• Ethanol precipitation
• Ligation
• Transformation to E.coli
• Transformation to yeast
• Cell lysis
• Yeast genomic DNA extraction
• Western blotting
• GFP testing