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From 2009.igem.org

1.Min prep
result of nano drop

sample       conc.
  45A        275 ng/uL
    B        319
  46A        212
    B        302
  47A        146
    B        181

2.digestion

K204027

Vector		Insert
1-6I	3	19	9
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH2O	13	dH2O	7
total	20uL	total	20uL

K204028

Vector		Insert
2-18H	3	2-16N	8
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH2O	13	dH2O	8
total	20uL	total	20uL

Vector		Insert
2-18F	2	1-19B	5
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH2O	14	dH2O	11
total	20uL	total	20uL

Vector		Insert
1-2M	5	1-14L	9
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH2O	11	dH2O	4
total	20uL	total	20uL

Vector		Insert
1-14N	10	1-8K	2
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH2O	6	dH2O	14
total	20uL	total	20uL

Vector		Insert
1-1D	5	33	5
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH2O	11	dH2O	10
total	20uL	total	20uL

K204051

Vector		Insert
1-6I	10	49	6
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH2O	6	dH2O	10
total	20uL	total	20uL

↓
37°C, 2hr
↓
gel electrophoresis
gel cut

4.colony check

  No.       no. of colony
  29            6
  38            1

5.digestion

K204045

Vector		Insert
31	12	1-8I	12
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH2O	4	dH2O	4
total	20uL	total	20uL

K204046

Vector		Insert
32	12	1-8K	12
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH2O	4	dH2O	10
total	20uL	total	20uL

↓
37°C,O/N

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