September/25 September 2009

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1.colony check

sample no.   no. of colony
  2-18B          >50
   53            >50
   X5            >50
   X6            >10
   X7             2
   X8             5

2.min prep
result of nano drop.

sample no.      conc.
   11          309 ng/uL
  3-1M         14
   28          260
   29          241
   30          219
   39          188
   46          224
   52          218
  X4-A         196
  X4-B         184

3.transformation

sample no. 37

4.digation

K204054

Vector		Insert
2-16L	10	28	3
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	6	dH₂O	13
total	20uL	total	20uL

K204055

Vector		Insert
29	4	28	6
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	12	dH₂O	10
total	20uL	total	20uL

K204056

Vector		Insert
1-23L	12	30	3
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	4	dH₂O	13
total	20uL	total	20uL

↓
37degree , 3hr
Agarose Gel Electro-phoresis
↓
gel cut
purification
ligation

5.transformation

sample no
  55 , 54

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