"
September/26 September 2009
From 2009.igem.org
1.colony check
sample no no of colony 37 0 54 + 55 +
2.min prep
result of nano drop
sample no. conc. X5 121 ng/uL X6 121 X7 209 X8 189 2-18B 137 53 7.8
3.ligation check
K204036
| Vector | Insert | ||
|---|---|---|---|
| 1-1F | 3 | 1-12M | 10 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 12 | dH2O | 6 |
| total | 20uL | total | 20uL |
K204037
| Vector | Insert | ||
|---|---|---|---|
| 1-1F | 3 | 1-8K | 5 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 12 | dH2O | 11 |
| total | 20uL | total | 20uL |
K204038
| Vector | Insert | ||
|---|---|---|---|
| 1-1F | 1 | 1-8I | 16 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 15 | dH2O | 0 |
| total | 20uL | total | 20uL |
K204039
| Vector | Insert | ||
|---|---|---|---|
| 1-1F | 3 | 2-8E | 8 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 12 | dH2O | 8 |
| total | 20uL | total | 20uL |
K204053
| Vector | Insert | ||
|---|---|---|---|
| 1-1F | 3 | 33 | 12 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 12 | dH2O | 4 |
| total | 20uL | total | 20uL |
↓
37degree , 3hr
Agarose Gel Electro-phoresis
↓
gel cut
purification
ligation
4.transformation
sample no. 55 , X4-A , 33 , 1-12E
